CHCHD4 is differentially expressed in central nervous system metastasis in human breast cancer.
Mamoor S (2023). CHCHD4 is differentially expressed in central nervous system metastasis in human breast cancer.., https://doi.org/10.31219/osf.io/8fmj3
- Overall rating
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(4.0) 1 review
- Authors
- Shahan Mamoor
- First published
- 2023
- Type
- Preprint
- DOI
- 10.31219/osf.io/8fmj3
Abstract
Metastasis to the brain occurs in a significant fraction of patients with breast cancer (1-3). We mined published RNA-sequencing and microarray data (4, 5) to compare primary and metastatic tumor transcriptomes for the discovery of genes associated with brain metastasis in humans with metastatic breast cancer. We found that coiled-coil-helix-coiled-coil-helix domain-containing 4, encoded by CHCHD4, was among the genes whose expression was most different in the brain metastases of patients with metastatic breast cancer as compared to primary tumors of the breast. CHCHD4 mRNA was present at increased quantities in brain metastatic tissues as compared to primary tumors of the breast. Expression of CHCHD4 in primary tumors, however, was not correlated with patient distant metastasis-free survival. Modulation of CHCHD4 expression may be relevant to the biology by which tumor cells metastasize from the breast to the brain in humans with metastatic breast cancer.
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Short and sweet - in this paper already published and available RNA-seq data is mined to compare differential gene expressions between primary breast cancer tumours and central nervous system metastases, in an effort to establish a link between this common occurrence. Differential CHCHD4 expression was identified and found to be statistically significant. Although CHCHD4 expression in brain metastases is significantly increased, there is no correlation with patient outcomes. The datasets used have small n numbers, but appropriate statistical analysis is conducted and all working shown. Charts not formatted, but this could be due to user download error, but it would've been nice to see some more graphs/figures regarding gene expression. This research justifies the use of murine models to further explore these findings in the context of breast cancer treatment and prevention of secondary tumour growth in the CNS. This paper is a great example of a seemingly small and simple project, with much larger implications. All working is shown and the story is clearly told with focus.